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. 2017 Aug 11;7:7903. doi: 10.1038/s41598-017-08415-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative histology for an S. aureus infected mouse sampled from deltoid muscle, using Gram-staining (left image) and hematoxylin & eosin (H&E, center and right image). In live inoculations, scattered inflammatory cells and intact bacteria are seen, best identified by Gram-staining and denoted by arrowheads. In heat-killed inoculations (right), several inflammatory cells are present on H&E staining without discernible bacteria. Images are representative of four animals.