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. 2017 Aug 11;7:7980. doi: 10.1038/s41598-017-08792-3

Figure 1.

Figure 1

NTP treatment reduces proliferation of MDA-MB-453, MDA-MB-231 and MCF10A cells in a time dose dependent inhibition. (A) Schematic of the non-thermal atmospheric plasma set-up utilised in this study, and a photograph of the air plasma. All cells were exposed to NTP for 0, 60, 90 or 120 s. Forty-eight hours later, cell images were captured with 100 × magnification (B) and cell numbers of each dish were counted by an automatic analyzer CountStar (C). All experiments were replicated a minimum of three times. Data are presented as means ± S.D. and statistical analysis was carried out using one-way ANOVA with Tukey’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001 versus control).