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. 2017 Aug 11;7:7980. doi: 10.1038/s41598-017-08792-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of NTP treatment with or without ROS inhibitor NAC on MDA-MB-453, MDA-MB-231 and MCF10A cells proliferation. Cells were treated with (PRE NAC and POST NAC) or without NAC (NTP) exposed to NTP for 0, 60, 90 or 120 s. Forty-eight hours later, cell numbers of each dish were counted by an automatic analyzer CountStar. ROS fluorescence was analyzed by FACSVerse flow cytometer, 1 h after 120 s NTP treatment. Cells were treated with: Negative Control (NC), 120 s NTP treatment (NTP), pretreat with 5 mM NAC before 120 s NTP treatment (NTP + PRE NAC), post-treat with 5 mM NAC immediately after 120 s NTP treatment (NTP + POST NAC). For improved illustration, a guidance ratio (compared with NC group) has been indicated through histograms. All experiments were replicated a minimum of three times.