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. 2017 Aug 11;7:7980. doi: 10.1038/s41598-017-08792-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of 120 s NTP treatments with extracellular and intracellular ROS inhibitor on cells’ proliferation. MDA-MB-453 (A), MDA-MB-231 (B) and MCF10A (C) cells were treated: Negative Control (NC), 120 s NTP treatment (NTP) without or with 500 U/ml catalase (NTP + Catalase). MDA-MB-231 cells (D) were treated: exposed to NTP for 0 and 120 s, pretreat with 5, 6, 10 and 12 mM NAC before 120 s NTP treatment. After forty-eight hours, cell numbers of each dish were counted by an automatic analyzer CountStar. All experiments were replicated a minimum of three times. Data are presented as means ± S.D. and statistical analysis was carried out using one-way ANOVA with Tukey’s multiple comparison test (NS = No significance, *p < 0.05, **p < 0.01, ***p < 0.001).