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. 2017 Aug 11;7:7980. doi: 10.1038/s41598-017-08792-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of NTP on the IL-6R/pSTAT3 and HER2/pAkt pathway in MDA-MB-231 and MCF10A cells. MDA-MB-231 (A) and MCF10A (B) cells were exposed to NTP for 0, 60, 90 or 120 s. After forty-eight hours post-treated with 5 mM N-acetyl cysteine (NAC+) or without (NAC−), proteins from total cell lysates were harvested. The expressions of HER2, L-6R, pSTAT3, tSTAT3, PTEN, pAkt and tAkt were detected by Western blot analysis. GAPDH was taken as a loading control throughout. The gels/blots were processed under the same experimental condition. Cropped gels/blots are displayed and full-length blots/gels are presented in Supplementary Figure 5.