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. 2017 Aug 11;7:7927. doi: 10.1038/s41598-017-08513-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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2-ME interventions for 4 weeks ameliorate glucose tolerance defects in chronic HFD induced-insulin-resistant mice. (a–d) Body weight, liver weight, epididymal weight and fasting blood glucose levels were analyzed in control, HFD, and 2-ME intervened HFD mice. N = 8 were analyzed in each group. (e) The IPGTT analysis. N = 8 from each group were analyzed. (f) Measurement of AUC value of glucose. (g) Insulin level. N = 8 were analyzed in each data set. (h) AUC value of insulin. (i) Insulin resistance index. The data in the figures are shown as the mean ± s.e.m. N = 8 were analyzed in each data set. The Mann-Whitney test was carried out to determine of statistical significance. Prism7.0 software was utilized for the statistical calculation. The mice fed the control diet are designated as “control”, whereas the mice fed the HFD are designated as “HFD”.