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. 2017 Aug 11;7:7927. doi: 10.1038/s41598-017-08513-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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COMT siRNA-mediated silencing introduces the features of type 2 diabetes and metabolic syndrome in HFD mice. (a,b) Western blot analysis of COMT and AMPK phosphorylation in the liver of scramble and COMT siRNA-injected mice. Scramble and COMT siRNA were injected intraperitoneally once weekly for 3 weeks at a dose of 10 mg/kg body weight. A representative image from 6 blots is shown. Cropped images were displayed and original blots are shown in the figure Supplementary 13. Densitometric data analysis is normalized to Actin. N = 6 were analyzed in each data set. (c,d) Body weight and organ weight measurements in the scramble and COMT siRNA injected mice. N = 6 were analyzed. (e) IPGTT analysis. N = 6 were analyzed in each data set. (f) glucose AUC value. (g,h) Insulin value at different time intervals (0, 15, 30 and 60 min post glucose load) with AUC. N = 6 were analyzed in each data set. (i) Insulin resistance index. N = 6 were analyzed in each data set. Data in the graph are shown as the mean ± s.e.m. Scramble siRNA was designated as scr siRNA whereas COMT siRNA was designated as COMTsiRNA in the figure. Prism7.0 software was utilized for the statistical calculation. The Mann-Whitney test was carried out to determine of statistical significance.