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. 2017 Aug 11;7:7976. doi: 10.1038/s41598-017-08348-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tissue-specific expression of STAiRs. RNA was isolated from various (A) cell lines and (B) tissues. DNase-digested RNAs as well as Ambion’s FirstChoice® Human Tissue Total RNAs were reverse-transcribed and subjected to standard PCR using primer pairs for primary STAiRs, resulting in product sizes of 264 bp for STAiR1, 253 bp for STAiR2, 271 bp for STAiR6, 252 bp and 625 bp for STAiR15.1 and STAiR15.2, respectively, as well as 330 bp and 106 bp for STAiR18.1 and STAiR18.2, respectively. Individual gel runs are indicated by gray boxes. Raw data images are displayed in Supplemental Fig. 2. (C) Differential expression of spliced STAiRs 15 and 18 transcripts in tumorous and according healthy control samples. DNase-digested total RNA was reverse-transcribed and subjected to qPCR using specific intron-spanning primers for STAiRs 15, 18, and STAT3. Expression values were normalized to U6 RNA and compared to the corresponding healthy control sample. Data are expressed as mean ± SD (n ≥ 3). STAiR18 expression was significantly (p < 0.05) elevated in every tumor sample tested.