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. 2017 Aug 11;7:7995. doi: 10.1038/s41598-017-08405-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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X-ray irradiation inhibited DNA synthesis in tumor cell lines in vitro. DNA synthesis (scale bar = 200 μm) was detected by 5-ethynyl-2′-deoxyuridine incorporation (EdU) incorporation assay in X-ray irradiated HepG2 cells after culturing for 24 h (A). DNA synthesis was detected by EdU incorporation assay in X-ray irradiated HepG2 (B), Huh7 (C), and SK-Hep1 (D) cells after culturing for 24 h and 72 h. DNA synthesis in these three cell lines exposed to 30 Gy and 50 Gy X-ray irradiation after culturing for 24 h is shown in panel E. Date are means ± SEM; n = 6. Con: the dose of irradiation was 0 Gy. **p < 0.01 vs. the control group at the same culture time; ^# p < 0.05, ^## p < 0.01 vs. the same treated group cultured for 24 h.