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. 2017 Aug 11;7:7768. doi: 10.1038/s41598-017-04737-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum-likelihood phylogenetic trees of ID1-DBL2x variants in (A) Malawi and (B) Benin. All trees were bootstrapped 1000x and bootstrap values >80 were used as cutoff points for significant branch differentiation. In Malawi, variants clustered in two distinct clades with the two referent strains (3D7 and FCR3). In Benin, in addition to the 3D7 and FCR3 clades, three other clades were detected.