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. 2017 Aug 11;7:7986. doi: 10.1038/s41598-017-08208-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of ZIP injections on the initial stage of neuropathic pain. To evaluate the effects of ZIP on the initial state of neuropathic pain, ZIP was injected into the ACC after neuropathic surgery. ZIP-injected rats showed a normal mechanical threshold up to 7 days after surgery. However, after the completion of the ZIP injection, the mechanical threshold decreased rapidly, which resulted in the development of neuropathic pain.