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. 2017 Aug 11;7:7992. doi: 10.1038/s41598-017-08703-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CREB3L2/BBF2H7 transport from the ER to the Golgi does not depend on Sec23A and Sec24D. (a) LX-2 cells starved for 24 h with DMEM supplemented with 0.5% FBS were treated with 1 ng/ml TGF-β1 for the indicated times. Proteins were extracted and subjected to SDS-PAGE, followed by western blotting with anti-CREB3L2/BBF2H7 and anti-GAPDH antibodies. (b) LX-2 cells transfected with the indicated siRNA(s) were cultured for 24 h in DMEM supplemented with 10% FBS. After starvation for 24 h with DMEM supplemented with 0.5% FBS, the cells were treated with 1 ng/ml TGF-β1 and cultured for 48 h. Proteins were extracted and subjected to SDS-PAGE, followed by western blotting with anti-CREB3L2/BBF2H7, anti-Sec23A, anti-Sec24D and anti-GAPDH antibodies. Shown are representative immunoblots (n = 3). Uncropped original blots were shown in Fig. S3.