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. 2017 Aug 11;7:7896. doi: 10.1038/s41598-017-08333-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential analysis with small sample sizes. The replicate LC-MS/MS runs of the AI 10 mM AAPH, AI reference and ADC reference samples were resampled to a size of 2 or 3 per condition (without replacement) prior to the differential analysis based on H ₀ ⁽¹⁾. X-axis: compared forms. Y-axis: statistical significance, FDR-adjusted p-values on the negative log10 scale. The horizontal lines indicate the 0.05 FDR cutoff. For each form, the vertical line represents the range between the maximum and minimum significance values among the testing results for the resampled data, and the dot represents the medium value. (a) The results for the positive controls (AI 10 mM AAPH versus AI reference). (b) The results for the negative controls (ADC reference versus AI reference). The list of compared forms for the negative controls is shown in Supplementary Table S6.