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. 2017 Aug 11;7:7884. doi: 10.1038/s41598-017-07385-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA transcript levels of PDHc subunits (A) E1α, (B) E1β, (C) E2 and (D) E3 in red muscle, white muscle and brain of crucian carp. X axes show treatment groups: normoxic control (N7), 1 day anoxia (A1), 7 days anoxia (A7) and reoxygenation (R). Left y-axes and bars show percentage distribution of paralogs, while right y-axes and filled circles show overall expression levels of genes (means ± S.E.M. of all paralogs combined; n = 6–8 fish per group). Significant difference compared to N7 is indicated by * for percentage distribution data and # for overall expression. */^#P > 0.05; **/^##P > 0.01; ***/^###P > 0.001 (One-way ANOVA; Holm-Sidak post-hoc test).