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. 2017 Aug 11;7:7885. doi: 10.1038/s41598-017-07392-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of heterotrimeric G protein α subunits gene expression levels in hypertrophic cardiomyocytes. Comparative analysis of Gα subunit gene expression of the four G protein families (G_i/o, G_q/11, G_s, G_12/13) in total RNA extracts from cardiomyocytes isolated from mice exhibiting or not (control) transversal aortic banding (TAC) obtained by Fluidigm Real-time qPCR. Results are expressed as the relative induction of the genes in C57Bl/6 mice submitted to TAC for 15 days (n = 6) compared to control mice (n = 6) wherein genes expression are equal to 1. The statistical significance of the change in expression level was assessed using unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.