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. 2017 Aug 11;7:7958. doi: 10.1038/s41598-017-08483-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NK cell cytotoxicities to cisplatin-resistant lung cancer cells vs. parental cells. (A,B) LDH-release based NK cell cytotoxicity tests (A), with NK92 cells; (B) with primary NK cells). A549P/A549CisR and H157P/H157CisR cells were plated and on the next day either NK 92 cells (A) or primary NK cells (B) were added at various ratios (in triplicate). Media (50 μl) was collected after 4 hours of tumor cells/NK cells co-culture and the LDH release was measured according to the manufacturer’s instruction. (C) Colony formation assay. A549P/A549CisR and H157P/H157CisR cells were plated and NK cells were added similarly as in (A and B). Media was changed into normal media after 4 hours of tumor cells/NK cells co-culture, survived cells were cultured until colonies become visible, stained with Crystal Violet, and colony numbers were counted under microscope. *p < 0.05, **p < 0.01, ***p < 0.001.