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. 2017 Aug 11;7:7958. doi: 10.1038/s41598-017-08483-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of neutralizing Abs of PD-L1 or PD-1 on the susceptibilities of cisplatin-resistant lung cancer cells to NK cell cytotoxicity. (A,B) NK cytotoxicity tests to A549P/A549CisR and H157P/H157CisR cells upon addition of anti-PD-L1 Ab to tumor cell/NK cell co-culture. (A with NK92 cell line; B with primary NK cells) (C). NK cell cytotoxicity tests to A549P/A549CisR and H157P/H157CisR cells upon addition of anti-PD-1 Ab to tumor cell/NK cell co-culture (with NK92 cells). (D) Western blot analyses showing PD-L1 levels in A549P/A549CisR and H157P/H157CisR cells after incubation with either control IgG or PD-L1 Ab. *p < 0.05, **p < 0.01, ***p < 0.001.