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. 2017 Aug 11;7:7867. doi: 10.1038/s41598-017-08384-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression pattern analysis of the bovine SIX1. (a) Analysis of the bovine SIX1 expression pattern in tissues and organs. (b) Expression pattern of the bovine SIX1 mRNA at different developmental stages. (c) Bovine Six1 expression pattern at different developmental stages. The samples of the longissimus thoracis were obtained at 1, 3, 6, 12, 18, 24, 36 and 48 months after birth. SIX1 mRNA expression was normalized to the housekeeping gene GAPDH and the expression levels were calculated relative to the gene expression in the liver and 24 months, respectively. The value of each column represents the mean ± standard deviation of three independent experiments. The unpaired Student’s t-test was used to detect significant differences. “*”P < 0.05 and “**”P < 0.01.