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. 2017 Aug 11;7:7867. doi: 10.1038/s41598-017-08384-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results of 5′RACE analysis of the SIX1 cDNA from skeletal muscle. (a) Products of the 5′ RACE of the SIX1 (arrow) from nested PCR were analysed by agarose gel electrophoresis. (b) Sequence of the SIX1 mRNA region. The primers (R1 and R2) were used for the 5′ RACE analysis are underlined. The positions of identified TSS are marked with arrows and red underline. The translational start site (ATG) is shown in red letters.