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. 2017 Aug 11;7:7867. doi: 10.1038/s41598-017-08384-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Constitutive activities of the bovine SIX1 haplotypes in QCMCs. The relative lucferase of the transcriptional activities in the candidate haplotypes was used to normalize the promoter activity. Haplotypes of the proximal minimal promoter region of the SIX1 gene were transfected into the QCMCs, and pGL3-Basic served as a control. The cells were collected for the luciferase assay at 48 h. The results are expressed as the mean ± standard deviation in arbitrary units based on the firefly luciferase activity normalized to the renilla luciferase activity in triplicate transfections. The unpaired Student’s t-test was used to detect significant differences. “*”P < 0.05 and “**”P < 0.01.