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. 2017 Aug 11;7:7908. doi: 10.1038/s41598-017-08243-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of zygotic transcripts by qRT-PCR in embryos with altered nuclear size and ploidy. One-cell diploid and haploid embryos were microinjected with mRNAs to increase (a) or decrease (b) nuclear size and allowed to develop to 4.5 hpf (a) or 7.5 hpf (b). Total RNA was isolated from 30 embryos for each condition and converted to cDNA. Expression levels of three zygotic genes (xnr5-13, xnr3, and bix1.1) were determined by qRT-PCR, normalized to ODC. Gene-expression levels are plotted in arbitrary units (AU) relative to control diploid embryos. The means from two independent experiments are shown. Error bars represent SD. ***p < 0.001; **p < 0.01; *p < 0.05; NS = not significant.