Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 11;7:7972. doi: 10.1038/s41598-017-08062-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of piperine analogs on Rho 123 accumulation in (a) KB 3–1 (parental) and KB Ch^R 8–5 (resistant) cells and (b) SW480 (parental) and SW480-VCR (resistant) cells. Cells were incubated with 4 μM of verapamil, piperine, Pip1, and Pip2. The compounds concentration in this assay was twice as that used for the cell viability and clonogenic assay, so as to clearly differentiate the Rho 123 accumulation between the control and treated cells. All the images were acquired using 20 × objective in a Nikon Eclipse Ti-S inverted fluorescence microscope (Scale 100 µM) (Nikon Instruments, Melville, New York), and processed with Nikon Br 4.0 Software.