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. 2017 Aug 11;7:7937. doi: 10.1038/s41598-017-08465-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptional response of caffeine biosynthetic genes to elevated CO₂ in tea leaves. Tea seedlings were exposed to either ambient (380 µmol mol⁻¹) or elevated CO₂ concentration (800 µmol mol⁻¹) for 24 days. Expression levels of genes were analyzed by qPCR using gene-specific primer pairs (Supplementary Table S1). Four biological replicates were used for qPCR analysis.