Fig. 7.

CK2 and CaM regulate the basolateral Cl⁻/HCO₃ ⁻ exchanger via similar signalling pathways: Calu-3 cells were loaded with BCECF-AM (10 μM; 1 h) and pH_i was determined by fluorescent microscopy. The activity of the basolateral Cl⁻/HCO₃ ⁻ exchanger was assessed by measuring pH_i changes in response to replacement of basolateral Cl⁻ with gluconate. a and b display the effect of TBB (10 μM; 5 min exposure), J-8 preincubation (50 μM, 1 h) and J-8 preincubation + acute TBB treatment on the activity of the basolateral Cl⁻/HCO₃ ^- exchanger, assessed by measuring the mean change in pH_i caused by basolateral Cl⁻ removal and the rate of reacidification upon Cl⁻ readdition respectively. ***Significant effect of treatment (p < 0.001); N.S. non significant (p > 0.05). Data represents mean ± S.E.M., n = 3–6