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. 2017 Aug 1;174(17):2929–2940. doi: 10.1111/bph.13924

Figure 1.

Figure 1

Specific expression of eGFP in PVN‐CRH neurons. (A) A construct of AAV vector containing an eGFP sequence driven by the rat CRH promoter. WPRE, Woodchuck post‐regulatory element; ITR, inverted terminal repeat; bGH, bovine growth hormone; polyA, polyadenylation. (B) eGFP expression in the PVN neurons induced by microinjection of AAV‐CRH promoter eGFP into the PVN. (C) Immunostaining images showing that eGFP‐tagged neurons (green) are CRH immunopositive. Scale bars in (C) indicate 50 μm.