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. 2017 Aug 10;8:1406. doi: 10.3389/fpls.2017.01406

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Copyright © 2017 Jia, Du, Ding, Zhang, Xing, Wei, Zhao, Mao, Li, Li and Jia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of MdFERL1, SlFERL1, and MdFERL6 on the expression of ripening-related genes. (A) Effect of MdFERL1 and MdFERL6 on the expression of tomato ripening-related genes. The MdFERL1-OE (overexpression) and MdFERL6-OE constructs were injected into tomato fruits at 18 DPA and gene expression was analyzed 10 days after transfection. Control samples were transfected with the empty vector (pCambia1304). RT-qPCR was conducted using SlACTIN as an internal control. Values are means + SD of three biological replicates. Double asterisks denote a significant difference at ^**P < 0.01 and ^*P < 0.05 using Student's t-test. (B) Effect of SlFERL1-VIGS (virus-induced gene silencing) on the expression of tomato ripening-related genes. The VIGS constructs were injected into fruits at 18 DPA, and gene expression was examined 12 days after transfection, using RT-qPCR. SlACTIN was used as the internal control. Values are means + SD of three biological replicates. Double asterisks denote a significant difference at ^**P < 0.01 and ^*P < 0.05 using Student's t-test. (C) Effect of MdFERL6-OE on the expression of ripening-related genes in apple calli. RT-qPCR was conducted using MdACTIN as an internal control. Values are means + SD of three biological replicates; different lowercase letters represent significant differences based on ANOVA (P < 0.05). (D) Effect of MdFERL6-AS and MdFERL6-AS combined with 1-MCP on the expression of ripening-related genes in apple calli. RT-qPCR was conducted using MdACTIN as an internal control. Values are means + SD of three biological replicates; different lowercase letters represent significant differences based on ANOVA (P < 0.05).