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. 2017 Aug 10;8:1406. doi: 10.3389/fpls.2017.01406

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Copyright © 2017 Jia, Du, Ding, Zhang, Xing, Wei, Zhao, Mao, Li, Li and Jia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ethionine activity assay. (A) Ethionine altered the growth status of apple calli. Apple calli transfected with empty vector (1); MdFERL6-AS (2); and MdFERL6-OE (3). (B) Measurement of calli weight. Transfected apple calli were weighed before treatment (0 day, and were then cultivated with ethionine for 7 days. The increase in weight between 0 and 7 days is indicated on the y-axis. Values are means + SD of three biological replicates. Single asterisks denote a significant difference at P < 0.05 using Student's t-test; double asterisks denote a significant difference at P < 0.01 using Student's t-test. (C) Gene expression of MdFERL6 and MdSAMS were detected in apple calli cultivated in 200 μM ethionine for 7 days. Values are means + SD of three biological replicates. Single asterisks denote a significant difference at P < 0.05 using Student's t-test; double asterisks denote a significant difference at P < 0.01 using Student's t-test.