Table 1.
Effects of SlFERL1-VIGS (virus-induced gene silencing) on major fruit ripening-related parameters.
| Parameters | CK | VIGS | Notes |
|---|---|---|---|
| Firmness (kg cm−2) | 9.067 ± 0.794 | 7.8 ± 0.825** | Cell wall metabolism-related parameter |
| Flavonoid content (μg g−1 FW) | 1.782 ± 0.056 | 1.508 ± 0.079** | Polyphenol metabolism-related compounds |
| Total phenol content (μg g−1 FW) | 4.37 ± 0.66 | 3.5 ± 0.31** | |
| Fructose content (mg g−1 FW) | 15.36 ± 1.613 | 14.51 ± 1.524 | Sugar metabolism-related compounds |
| Sucrose content (mg g−1 FW) | 9.925 ± 0.842 | 7.472 ± 0.694** | |
| Glucose content (mg g−1 FW) | 33.029 ± 1.964 | 37.59 ± 2.511** | |
| Total titratable acid content (%) | 2.578 ± 0.064 | 2.786 ± 0.123 | Acid metabolism-related parameter |
| Hydrazinecarboxamide | 1.535 ± 0.035 | 1.665 ± 0.048 | Aroma metabolism-related compounds (data are expressed as percentage of the total volatiles) |
| Acetaldehyde,hydroxy | 3.13 ± 0.062 | 2.3 ± 0.0452 | |
| Silanediol, dimethyl | 1.19 ± 0.086 | 1.855 ± 0.071** | |
| 2-Pentenal, (E) | 0.175 ± 0.021 | 0.3 ± 0.056 | |
| 2-Pentenal, (E) | 1.985 ± 0.079 | 3.41 ± 0.096** | |
| Oxime-,methoxy-phenyl | 0.18 ± 0.056 | 0.435 ± 0.036** | |
| Heptanal | 0.2 ± 0.004 | 0.11 ± 0.014 | |
| α-Pinene | 0.7 ± 0.003 | 0.53 ± 0.005** | |
| 2-Heptenal,(Z) | 0.615 ± 0.063 | 0.575 ± 0.017 | |
| 5-Hepten-2-ol, 6-methyl | 0.29 ± 0.008 | 0.275 ± 0.021 | |
| D-Limonene | 0.1 ± 0.002 | 0.87 ± 0.007** | |
| 1-Octanol | 0.265 ± 0.014 | 0.36 ± 0.005 | |
| 1,6-Octadien-3-ol,3,7-dimethyl | 0.13 ± 0.005 | 0.21 ± 0.009 | |
| Nonanal | 0.255 ± 0.002 | 0.385 ± 0.009 | |
| Methyl Salicylate | 1.86 ± 0.042 | 2.285 ± 0.032** | |
| 2-Decenal,(E) | 0.11 ± 0.004 | 0.16 ± 0.007 | |
| 2,6-Octadienal,3,7-dimethyl | 0.19 ± 0.002 | 0.15 ± 0.008 | |
| Tetradecane | 0.105 ± 0.035 | 0.13 ± 0.006 | |
| Hexadecane | 0.245 ± 0.007 | 0.275 ± 0.006 | |
| Phenol,2,4-bis(1,1-dimethylethyl) | 0.008 ± 0.001 | 0.1 ± 0.003** | |
| Dodecanoic acid, ethyl ester | 0.07 ± 0.002 | 0.23 ± 0.006** |
The SlFERL1-VIGS construct was transfected into tomato at 18 days post-anthesis. At 12 days after transfection, fruits were detached and analyzed. Values are means ± SD of two samples (each sample includes five fruits). Asterisks denote significant differences compared with the control sample (i.e., VIGS-C) at P < 0.01, using Student's t-test. C, control; FW, fresh weight.