Skip to main content
. 2017 Aug 10;11:241. doi: 10.3389/fncel.2017.00241

Figure 4.

Figure 4

AF-6 overexpression rescues the disease phenotypes of leucine rich repeat kinase 2 (LRRK2) mutant flies. (A) Climbing score and (B) DA neuronal count (PPL1 cluster) of control (yw), Ddc/+, UAS-AF-6/+ and LRRK2 G2019S (GS) mutant flies in the absence or presence of AF-6 overexpression using Ddc Gal4 driver at day 50 post eclosion. (C) Relative DA levels in these flies as measured by the HPLC. (D) Bar-graph showing the average size ± SEM of mito-GFP punctae in control (yw), GS mutant flies and GS/AF-6 double mutant flies at day 50 post eclosion. (E) Representative confocal microscopy images showing the localization of mito-GFP (green) in TH-positive neurons (red) of control (yw), GS mutant flies and GS/AF-6 double mutant flies. *p < 0.05, **p < 0.001; unpaired student’s t test.