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AF-6 overexpression mitigates the neurotoxicity of rotenone. (A) Climbing score and (B) Bar graph showing the number of TH-positive DA neurons (PPL1 cluster) of control Ddc/+ flies (treated with 0.5% DMSO), Ddc/+ and UAS-AF-6/+ after 15 days of exposure to 50 μM rotenone treatment using the Ddc Gal4 driver. (C) Representative confocal microscopy images showing TH-positive (red) DA neurons in the PPL1 cluster. *p < 0.05, **p < 0.001; unpaired student’s t test.
