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. 2017 Aug 10;8:968. doi: 10.3389/fimmu.2017.00968

Figure 3.

Figure 3

Polyfunctional capacity of early secretory antigenic target (ESAT)-6/culture filtrate protein (CFP)-10- and cytomegalovirus virus (CMV)-specific CD4+ T cells in individuals with distinct HIV and tuberculosis (TB) disease status. (A) Representative dot plots of the expression of TNFα, IFNγ, and IL-2 from CD4+ T cells after stimulation with ESAT-6/CFP-10 or CMV peptide pool in one latent tuberculosis infection (LTBI)/HIV− participant. NS corresponds to unstimulated PBMC. The frequency of cytokine-producing cells expressed as a percentage of the total CD4+ T cell population is indicated. (B,C) Graphs and pie charts representing the polyfunctional profile of CD4 T cells in response to ESAT-6/CFP-10 (B) and CMV (C). Each section of the pie chart represents a specific combination of cytokines, as indicated by the color at the bottom of the graph. The black arc on the pies corresponds to IL-2-producing cells. Horizontal bars depict the median with interquartile ranges indicated. Statistical comparisons were performed using a Wilcoxon rank-sum test. *p < 0.05, **p < 0.01, ***p < 0.001. (D,E) Relationship between the CD27 expression on antigen-specific IFNγ+ CD4+ T cells and the functional capacity of antigen-responding CD4+ T cells in HIV-uninfected individuals with latent and active TB (aTB). Correlations were tested by a two-tailed non-parametric Spearman rank test.