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. 2017 Aug 10;10:244. doi: 10.3389/fnmol.2017.00244

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Copyright © 2017 Pacheco, Aguayo, Aliaga, Muñoz, García-Rojo, Olave, Parra-Fiedler, García-Pérez, Tejos-Bravo, Rojas, Parra and Fiedler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of chronic stress on AMPA subunit levels in synaptosomal fraction of DH and VH. (A) Morphological characterization of synaptosomal fraction by electron microscopy. Photograph of the synaptosomal fraction; shows the presynaptic button and the presynaptic vesicles attached at the postsynaptic density. Picture was taken with 105,000× magnification. (B) Biochemical characterization of the different fractions obtained during synaptosome preparation. H represents the hippocampal homogenate, N represents nuclear fraction and Syn represents the synaptosomal fraction. Markers were evaluated by immunoblot. The nuclear marker (LAP-2a) was not detected in synaptosomal fraction. The PSD-95 (Post-synaptic marker), GluA1 and NR1 subunits of AMPA and NMDA receptor (NMDAR), respectively were enriched at the synaptosomal fraction. Levels of GluA1 and GluA2 subunit were evaluated in synaptosomal fraction obtained from dorsal (C) and ventral (D) hippocampus. Upper panel shows a representative western blot. Graphs represent mean ± SEM of each group. Number of animals is inside bars.