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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Radiat Res. 2017 May 2;188(1):35–43. doi: 10.1667/RR14620.1

FIG. 2.

FIG. 2

The effects of gossypol on extracellular matrix. Mice were irradiated and vehicle or gossypol treated as described in Materials and Methods. At week 26 postirradiation, the right middle lung lobe was collected for RNA isolation and mRNA expression analysis by qRT-PCR. mRNA quantification is expressed as fold change from nonirradiated controls normalized to 18S for col1a1 (panel A), col3a1 (panel B) and fibronectin (panel C). Total right lung homogenates were analyzed for native collagen using a slot blot (panel D), and for fibronectin by Western blot (panel E). Fibronectin protein was quantified using densitometry (panel F). Data are shown as mean ± SEM for n = 5–8 mice per group. *P ≤ 0.05 compared to nonirradiated vehicle-treated controls; #P ≤ 0.05 compared to irradiated vehicle-treated mice (ANOVA).