Fig. 3.
Growth of wild-type strain and Δlrp mutant in mouse blood as well as serum with or without supplementation of iron sources. a The bacteria (6 × 105 cfu/ml) were incubated in mouse serum or whole blood at 37 °C. b The bacteria (6 × 105 cfu/ml) were incubated in mouse serum without supplementation or supplemented with hemin (hm; 10 μM), hemoglobin (hg; 10 μM) and ferric ammonium citrate (FAC:;200 μg/ml), respectively. The viable bacteria were enumerated by viable counts at the indicated time points. n = 3. YJ016: wild-type strain; YH01: Δlrp mutant. The significance of difference was analyzed by two-way ANOVA along with Bonferroni’s post test. **: P < 0.01; ***: P < 0.001 for the difference between the results obtained at 6 h after incubation