Table 1.
Bacterial strains and plasmids used in this study
| Strain/Plasmid | Description | Source/Reference |
|---|---|---|
| Escherichia coli | ||
| S17-1λpir | Thi thr leu tonA lacY supE recA::RP4–2 (Km::Tn7,Tc::Mu-1) lysogenized with λpir | [21] |
| KA014 | BL21(pMO08) | This study |
| WT29 | ArcticExpress(pMO08) | This study |
| Vibrio vulnificus | ||
| YJ016 | Clinical isolate from blood | [30] |
| NY303–2 | YJ016∆vpl∆vvhA | This study |
| NY303 | Spontaneous attenuated mutant of NY303–2 | This study |
| YH01 | YJ016∆lrp, Cmr | This study |
| YH02 | NY303–2∆lrp, Cmr | This study |
| YH03 | YH01(plrp) | This study |
| YH04 | YH02(plrp) | This study |
| YH05 | NY303(plrp) | This study |
| YH06 | YJ016 containing lrp* cloned from NY303 | This study |
| CH08 | NY303–2 containing lrp* cloned from NY303 | This study |
| CS9133 | Clinical isolate (Biotype 1) | [21] |
| CH09 | CS9133Δlrp, Cmr | This study |
| CECT4999 | Eel pathogen (Biotype 2) | [31] |
| CH10 | CECT4999Δlrp, Cmr | This study |
| KA023 | YH01(pYU01) | This study |
| WT36 | YJ016 integrated with Plrp-lacZ in lacZ | This study |
| WT37 | YH01 integrated with Plrp-lacZ in lacZ | This study |
| WT38 | YH06 integrated with Plrp-lacZ in lacZ | This study |
| HL128 | YJ016∆rtxA1 | [18] |
| CP212 | YJ016∆lacZ | [44] |
| Plasmid | ||
| pUC19 | Cloning vector; Apr | [65] |
| pCVD442 | Cloning vector, mob RP4, sacB, and Apr | [66] |
| pIT009 | pJRD215 derivative with the Smr gene replaced by the MCS-containing lacZ gene cloned from pUC19 | [31] |
| plrp | pIT009 inserted with lrp cloned from strain YJ016 | This study |
| pKCW01 | pBBR1MCS4 inserted with mazEF cloned from pR99 at XbaI site | This study; [31, 67] |
| pYU01 | pKCW01 inserted with lrp of strain YJ016, T7 terminator and His6-tag | This study |
| pMO08 | pET30a(+)inserted with lrp cloned from strain YJ016 | This study |
| pMO15 | pCVD442 inserted with Plrp-lacZ | This study |
Km kanamycin-resistance gene, Tc tetracycline- resistance gene, Cm r chloramphenicol-resistant, Ap r ampicillin-resistant, Sm r streptomycin-resistance, MCS multiple-cloning site