Fig. 4.

Effects of the various Chinese herbal extracts (CHE) on the gene expression levels of the BDNF autoregulation loop using MDA-MB-231 line. Cultured MDA-MB-231 cells without/with each CHE individual (1 μg/mL for each herbal extract) pretreatment were lysed and the gene expression levels of BDNF and TrkB at both the protein and mRNA level were analyzed by Western blot (a) and real-time PCR (b), respectively. Data are expressed as mean + SEM. *, p < 0.05 compared to vehicle group by Mann-Whitney U test (n = four or five independent experiments in each group)