Figure 4.

Neoplasia is associated with high lactate and taurine levels in mouse tissue samples. (A) Representative CPMG spectra of pancreata from a wild type mouse treated with PBS, a wild type mouse treated with LPS, and an 8 week old Kras mouse. Spectra display increases in lactate and taurine and a decrease in tCho associated with oncogenic Kras. Spectra include peaks for phosphocreatine, glycine, and choline. All spectra illustrate normalization to sample weight. The 3.60–3.80 ppm region was cut to remove the residual ethanol signal resulting from the use of ethanol in collecting tissue samples. (B) Average levels of lactate, taurine, and tCho as determined from ¹H HR-MAS of pancreatic tissue samples of PBS-treated wild type mice, LPS-treated wild type mice, 4 week old Kras mice, and 8 week old Kras mice. Lactate and taurine levels are significantly higher and tCho levels are significantly lower in 8 week old Kras mice compared with PBS-treated wild type mice. *p < 0.05, **p < 0.0005.