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. 2017 Aug 10;10:239. doi: 10.3389/fnmol.2017.00239

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Copyright © 2017 Liu, Schrott-Fischer, Glueckert, Benav and Rask-Andersen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal and super-resolution (SIM) microscopy of the suprastrial space (SS). (A) Insulating type V fibrocytes express Claudin-11. The left inset shows cells facing the scala vestibuli (arrows). (B) Cx30 expression also reaches the SS reflecting conceivable transcellular recirculation of K⁺ from SV back to the StV. Laminin β2 stains the basal lamina of the RM and capillaries. (C) Polarized expression of NKCC1 and Na/K-ATPase (β1) in the StV and non-polarized manifestation in the suprastrial space may reflect the alternate directions of ion flow. Framed area is magnified in (D,E) and show expression of the NKCC1 and Na/K-ATPase (β1) membrane transporters facing SV. (F) The type V fibrocyte facing the SV shows both intracellular (^∗) and membrane expression (arrow) of Na/K-ATPase (SR-SIM). StV, stria vascularis; RM, Reissner’s membrane; V, stria vessel; SV, scala vestibuli.