FIGURE 6.

Super-resolution structured illumination microscopy of the RM/StV junction at the basal turn (single optical sections). (A) Na/K-ATPase (α1-subunit) and the Na/K/Cl cotransporter are highly expressed in the basolateral cell membrane of the marginal cells. The area in the upper box is magnified in the inset (top left). Na/K/Cl labeling appears together with Na/K-ATPase labeling in an alternating fashion. There is also some sub-membranous staining. Type V fibrocytes of the suprastrial space (SS) show high expression of the Na/K/Cl cotransporter and some Na/K-ATPase. Lower inset shows corresponding expression of Claudin-11 insulating the RM area. The area in the lower box is shown at a higher magnification in (B). (B) Large numbers of aggregates of Na/K-ATPase and Na/K/Cl protein are seen in the StV (shown at a higher magnification in D). (C) The boxed area in (B) is shown at a higher magnification. The type V fibrocytes in the SS contains aggregates of non-polarized Na/K-ATPase associated with the Na/K/Cl cotransporter.