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. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: Nature. 2017 May 17;545(7655):500–504. doi: 10.1038/nature22314

Figure 4. RNA binding protein MSI2 mediates BCAT1 activation in BC-CML.

Figure 4

a, b, RNA immunoprecipitation (RIP) with (a) anti-FLAG antibody from K562 cells expressing empty vector, FLAG-tagged MSI2 (WT) or FLAG-MSI2 with defective RNA binding domains (RBD), or (b) RIP with anti-MSI2 (αMSI2) or a control IgG (nIgG) from K562 cells. Co-immunoprecipitated RNAs were analyzed for the enrichment of BCAT1, beta-2-microglobulin (B2M) and c-MYC transcripts. n=3 each. c, d, Effect of (c) BCAT1 overexpression and (d) BCAA supplementation on the colony-forming ability of MSI2-knockdown K562 cells (n=3). (-), shCtrl or vehicle controls. e, Effect of nutrient supplementation on mTORC1 pathway activation in MSI2-knockdown K562 cells. f, Schematic model of the MSI2-BCAT1-BCAA axis in BC-CML. Error bars indicate s.e.m. *p<0.05, **p<0.01, by two-tailed t-test.