Chronic haloperidol treatment at 0.2 mg/kg/day, for 24 days, increased levels of 7DHC and 8DHC in the rat striatum, A, and prefrontal cortex, C. A trend toward increase was also observed at 0.05 mg/kg/day haloperidol, while chronic clozapine treatment had no effect. Patterns were similar if samples were normalized to protein (not shown), or to cholesterol levels. B, D, cholesterol levels (ng/μg protein) in striatum and prefrontal cortex, respectively. Gray boxes show non-parametric Anova data. ***p<0.0001 in post-hoc analysis using Dunnett’s comparison to control.