Figure 6.

Oncogenic H-Ras inhibits type I interferon signaling. (A) HEK293T cells were transfected with vector, HRASV12, or HRASN17 DNA at the indicated doses together with the pISRE-Luc reporter plasmid and the pTK-RL reporter plasmid. In all groups, the total amounts of transfected DNA were adjusted to the same using the control vector DNA. Cells were treated with or without IFN-α (1,000 U) at 24 h post-DNA transfection, and luciferase activities were measured at 24 h post IFN-α treatment. The expression levels of transfected H-Ras are shown on the right. (B) The RNA transcription levels of IFIT2, MX1, and OAS2 were determined by RT-qPCR and the induction folds were calculated as ΔΔCt. All the results of induction fold were obtained from three independent experiments and presented as the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 between the indicated groups and the vector-transfected group (one-way ANOVA followed by Dunnett’s post hoc analysis).