Figure 1.

Effects of MET18 deficiency on yeast response to oxidative stress. (a) mRNA expression of MET18 under unstressed condition was determined by real-time PCR. (b) Tenfold dilution series of WT and MET18/met18Δ cells were spotted on YPD plates with or without 0.03% MMS and then incubated at 30°C for 2~3 days to detect the cellular response to MMS. (c) Tenfold dilution series of WT and MET18/met18Δ cells were spotted on YPD plates with 0.225 mM CHP or 7 mM H₂O₂ and incubated at 30°C for 2~5 days to detect the cellular response to CHP and H₂O₂. Results shown are representative of three independent experiments, ^∗∗p < 0.01 versus WT cells (n = 3).