COS7 cells were cotransfected with plasmids encoding FLAG-tagged Nrdp1(CHSQ) and GFP-DP1 or CFP-Rtn4A, or with all three, as indicated. After 12 hours, the cells were fixed, stained, and imaged to show the FLAG epitope (red), CFP, and GFP. Scale bars, 10 µm. Data are representative of three independent experiments.