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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Inhal Toxicol. 2017 Jan;29(1):10–17. doi: 10.1080/08958378.2016.1271841

Figure 1.

Figure 1

Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all IgG (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - HRP for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p<0.001 by 2-tailed t-test.