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The overall morphology and extent of spreading of cells adherent to gels were not altered with increases in the E of the gels from 20 (a) to 60 (b) and 110 (c) kPa. Membranes were stained with octadecyl rhodamine B chloride to visualize cells. In contrast, formation of focal adhesions, as visualized with immunofluorescence localization of vinculin, was enhanced when E was increased from 20 (d) to 110 (e) kPa.
