Table 1.

Selected Important Points for Best Practices

Dissolving L-012 in saline appears to increase chances of precipitation of the probe. We recommend to dissolve it in distilled water. We recommend optimization of time points of imaging according to each injury model. Before image acquisition, it is important to observe the mouse eyes carefully using an ophthalmoscope to ensure that the pupil was fully dilated and there was no cataract or cornea abnormality. It is important to position the mouse head correctly so that the eye is directly facing the camera and to keep the cornea moist during the imaging procedure. It is important to minimize bleeding during or after surgery. Bleeding may cause activation of neutrophils, which produce large amount of superoxide and other free radicals, generating unnecessary noise to injury-induced signals. A sham-operated control group is important.