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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Mol Neurobiol. 2017 Feb 13;55(2):1676–1691. doi: 10.1007/s12035-017-0432-7

Figure 6. The effect of conditioned medium derived from cofilin transfected and LPS treated microglia on differentiated PC12 cells viability.

Figure 6

Microglial cells were transfected with scrambled/cofilin siRNA at 50 nM concentration for 72 h and then treated with LPS (100 ng/ml) for 24 h more. Afterwards microglial cells conditioned medium was added to differentiated PC12 cells and incubated for 24 h. MTT viability assay was done to assay the neurotoxic potential of the conditioned medium. Differentiated PC12 cells viability was significantly reduced by treatment with the conditioned medium derived from scrambled transfected and LPS activated microglia. However, cofilin knockdown in microglial cells protect differentiated PC12 cells from the neurotoxic effect of the conditioned medium. Data are expressed as mean±SEM of three independent experiments. **P < 0.01 relative to scrambled siRNA group without LPS treatment. ##P < 0.01 relative to scrambled transfected and LPS treated group.