Angle dependency of the probability of mechanical activation. (a) Probabilities of the mechanical activation were determined in the presence of 0.2 μM ATP (blue); 0.2 μM ATP, 30 mM Pi (green); 0.2 μM ATP, 1 mM ADP (red); and 0.2 μM ATP, 30 mM Pi, 1 mM ADP (purple). Each data point was determined from 17 observations on average. (b) Probabilities were determined in the presence of 0.2 μM ATP (blue), 2 mM ATP (black), and 33 μM ATP, 20 mM ADP (red). Each data point was determined from 15 observations on average.