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. 2005 Mar 7;102(12):4459–4464. doi: 10.1073/pnas.0501076102

Fig. 4.

Fig. 4.

cAMP stimulates distinct profiles of CREB target genes in different tissues. (a) Venn diagram showing overlap between CREB target genes in human islets, hepatocytes, and HEK293T cells. (b) ChIP-chip assay of promoters occupied by CREB and P-CREB in human hepatocytes compared with HEK293T cells. Top 1,000 and bottom 1,000 scoring genes from hepatocyte P-CREB ChIP-chip assays were plotted. Relative binding ratio observed for each gene is color-coded. (c) Comparison of P-CREB binding profiles from ChIP-chip assays of HEK293T cells, human pancreatic islets, and human hepatocytes. Top 200 and bottom 200 scoring genes from islet P-CREB ChIP-chip assays were plotted. (d) ChIP assay of HEK293T cells showing effects of FSK on recruitment of CBP to cAMP inducible (NR4A2) vs. noninducible (CDC37) genes. Levels of P-CREB on each promoter are indicated. (e) Quantitative PCR assay showing relative levels of CBP over cAMP inducible and noninducible promoters after 0, 1, or4hofFSK treatment by ChIP.